The low-toxic organic antifouling agents recognized internationally include Diuron, Irgarol 1051, Kathon 5287, TCMTB, and TCMS pyridine. This topical substance is also used as an agricultural pesticide. So far, organic biocides have been used alone as antifouling agents for ships. They are only used as antifouling agents for antifouling coatings such as copper and organotin, and are therefore called organic co-fouling agents. Copper compounds have antifouling activity against barnacles, pipeworms, and large algae, but are ineffective against several algae such as canola, water cloud, and bent algae. Therefore, the copper compound as an antifouling agent should be combined with the other organic antifouling agents at the same time, so that the ability to reach a broad spectrum of killing marine adhesion organisms in the antifouling paint.
2.1 Diuron, Irgarol1051
Irgarol 1051 manufacturers told us that Because diuron and Irgarol 1051 are only in the μg/L range in seawater and marine sediments, high-performance liquid chromatography with high acuity and low detection limit is used to determine such substances. GC can also measure diuron, but it needs to be pre-tested to prevent the decomposition of the heating car adapter before the measurement, and the HPLC rule eliminates the decoration step, which makes the measurement step easier . Diuron was importantly quantified by HPLC. There was a report on the determination of diuron in meat by HPLC-UV. The RP-C18 column was used. The flow phase methanol: water = 1:1, UV detection wavelength 254 nm. HPLC-PDA can be used for the detection of diuron and Cybutryne Irgarol. PDA is a kind of UV detector, which can not only obtain the chromatographic message, but also obtain the real photospectrum message of the compound. The new PDA detector is capable of high-sensitivity detection at 1.2nm high-European connector resolution, resulting in high-quality spectral messages . These qualities not only make it useful for high-sensitivity detection, but can be used for spectral recognition of compounds. In addition, it is also possible to collect a multi-channel 2D chromatogram, which is extremely suitable for optimizing the detection wavelength of a plurality of different residual substances. The use of diuron and Cybutryne as antifouling agents in seawater or sea mud is a major indicator of whether antifouling agents are toxic throughout the year. The content of diuron and Cybutryne CAS 28159-98-0 and its degradation products in the seawater and sediment of the US coupling was determined by HPLC-PAD method. Many honing literatures clarify that the products of photodegradation, biodegradation and chemical decomposition of Irgarol1051 are all M1 , and the degradation products of diuron under aerobic conditions include DCPMU, DCPU and DCA . The HPLC method was as follows: the HPLC conditions of seawater and sediment samples were similar. The Zirbax SB-C18 was added to the column and the extraction column. The column temperature was 30 ° C. The flowing phase formed into acetonitrile and water (MilliQ). The rheological phase was transformed in 25 min. The formation of the aviation plug was eluted with a gradient of 20% acetonitrile to 100% acetonitrile at a flow rate of 1.7 mL/min. The diode array detector set Irgarol 1051 and M1 to 224 nm, diuron and its metabolites to 244 nm. This attempt has achieved satisfactory recovery and detection limits for most of the substances in seawater and sediments .