Let us take a look at the synthesis of poly(3-mercaptopropionic acid 107-96-0) and poly(3-hydroxybutyric acid-3-mercaptopropionic acid) in recombinant Escherichia coli.
Using Clostridium acetobutylicum's butyric acid kinase gene (buk) and phosphotransbutyrylase gene (ptb), and Thiocapsa pfennigii's PHA synthase gene, a metabolic pathway capable of synthesizing various polyhydroxyalkanoic acids was designed. Transformation of E. coli to obtain recombinant E. coli strains. Previous studies have shown that under appropriate precursor conditions, the recombinant E. coli can synthesize various organisms including polyhydroxybutyric acid, poly(hydroxybutyrate-valeric acid) and the like. Polyester [Liu and Steinbüchel, Appl. Environ. Microbiol. 66: 739-743].
Using the recombinant Escherichia coli, a homo-copolyester of 3-mercaptopropionic acid was synthesized by biocatalysis, and a plurality of hetero-type copolymers containing 3-mercaptopropionic acid monomers were also obtained by using the recombinant Escherichia coli. The effect of 3-mercaptopropionic acid on the growth of Escherichia coli was optimized. On the basis of this, the timing and concentration of 3-mercaptopropionic acid added during the cultivation were optimized. The results showed that under the experimental conditions, the cells synthesized poly(3-mercaptopropane C). Acid) up to 6.7% (dry weight of cells), synthesis of poly(3-hydroxybutyrate-3-mercaptopropionic acid) (3-mercaptopropionic acid in the molecule: 3-hydroxybutyric acid = 3:1) up to 24.3 %. The experiment further studied the ability of recombinant E. coli synthetic polymers expressing the above three genes simultaneously or separately, and the results showed that the polymer could be synthesized only when three genes were simultaneously expressed, indicating that three genes are necessary for the synthesis process. This indicates that the synthetic route is carried out according to the designed route. The synthesized compounds have also been qualitatively studied by means of GC/MS, GPC, IR, etc. Poly(3-mercaptopropionic acid) or poly(3-hydroxybutyric acid) Polyesters such as -3-mercaptopropionic acid are a new class of biopolymers that are in the molecular skeleton. Containing a thioester bond, an ester bond different from the oxygen polyhydroxyalkanoate, thereby having significantly different physical properties, chemical, optical and other important potential applications.